Biofilm Management

Chronic wounds are frequently associated with diabetes, venous stasis, peripheral vascular disease and pressure ulcers. Although biofilms are estimated to cause 80% of all infections1, neither current standard clinical microbiology lab tests nor visual clinical indicators adequately confirm their presence2. Furthermore, current means of topically managing bioburden and biofilm, such as antiseptics (ie, Dakins solution, povidone iodine, PHMB), antimicrobials and topical antibiotics, are either ineffective at eliminating common pathogens when diluted to non-cytotoxic concentrations3 or unable to penetrate a mature biofilm4. The direct role of biofilms in delaying the healing cascade is not fully understood, however, the principles of wound bed preparation (ie. TIME principle) are vital when developing a treatment strategy5.

(235 mL) (40mL)

PhaseOne® and Wound Cleanser

A fast-acting microbicidal skin and wound cleanser, PhaseOne® penetrates and disrupts biofilm, killing bacteria and fungi, as well as neutralizes resulting toxins*. Formulated as a pure concentration of hypochlorous acid (HOCl) in saline, PhaseOne® is made without the toxic bleach and other preservative chemicals found in other products, making it gentle, non-irritating, and non-sensitizing to skin and new tissue.

PhaseOne® may be used for routine cleansing and irrigation and supports debridement of:

    • Intraoperative pocket lavage, before subcutaneous closure
    • Stage I to IV pressure injuries
    • Stasis ulcers
    • Leg ulcers
    • Diabetic foot ulcers
    • First-degree and second-degree burns
    • Post-surgical wounds
    • Grafted and donor sites
    • Minor burns, superficial abrasions, and wounds
    • Moistening absorbent wound dressings

    *=Results derived from in vitro testing of HOCl in solution. Data on file.

NPAK (Negative Pressure Accessory Kit):

Also known as the Prep-and-Close Kit, the NPAK offers the provider the flexibility and convenience of providing wound bed preparation and supporting wound closure. Consider using the NPAK:

  • Prior to initiating NPWT
  • To promote full closure in wounds that have sufficiently granulated, allowing for discontinuation of NPWT
  • As a convenient, cost effective, routine wound care system for weekly to biweekly wound care
  • To simplify stocking in the wound clinic and beyond

Biofilm in Saline

(Figure 1)

Biofilm in PhaseOne®

(Figure 2)

Most antimicrobial agents are incapable of penetrating mature biofilm. The unique chemistry of PhaseOne® (in solution)
rapidly penetrates and disrupts the biofilm matrix, targeting the bacteria within (Figure 2).

  1. Percival, Steven L, and Phillip G Bowler. “Biofilms and Their Potential Role in Wound Healing.” Wounds, vol. 16, no. 7, July 2004, pp. 234–240.,
  2. International Wound Infection Institute (IWII) “Wound Infection in Clinical Practice”. Wounds International. 2016.
  3. Rani, Suriani Abdul, et al. “The In Vitro Antimicrobial Activity of Wound and Skin Cleansers at Nontoxic Concentrations.” Adv Skin Wound Care, vol. 27, no.2, 2014, pp. 65–69., doi:10.1097/01.asw.0000443255.73875.a3.
  4. Mah, Thien-Fah C, and George A O’Toole. “Mechanisms of Biofilm Resistance to Antimicrobial Agents.” Trends Microbiol, vol. 9, no. 1, Jan. 2001, pp. 34–39., doi:
  5. Keast, David, et al. “Clinical Update TEN TOP TIPS Understanding and Managing Wound Biofilm.” Wounds International, vol. 5, no. 2, 2014, pp. 20–24.,